ParkinsonCanadaV1, Main, Exploration, bibRecord, 000376

Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells

Identifieur interne : 000376 ( Main/Exploration ); précédent : 000375; suivant : 000377

Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells

Auteurs : Sadek Shorbagi ; Ian R. Brown

Source :

Cell Stress & Chaperones [ 1355-8145 ] ; 2016.

RBID : PMC:5083669

Abstract

Heat shock proteins (Hsps) are cellular repair agents that counter the effects of protein misfolding that is a characteristic feature of neurodegenerative diseases. HSPA1A (Hsp70–1) is a widely studied member of the HSPA (Hsp70) family. The little-studied HSPA6 (Hsp70B’) is present in the human genome and absent in mouse and rat; hence, it is missing in current animal models of neurodegenerative diseases. Differentiated human neuronal SH-SY5Y cells were employed to compare the dynamics of the association of YFP-tagged HSPA6 and HSPA1A with stress-sensitive cytoplasmic and nuclear structures. Following thermal stress, live-imaging confocal microscopy and Fluorescence Recovery After Photobleaching (FRAP) demonstrated that HSPA6 displayed a prolonged and more dynamic association, compared to HSPA1A, with centrioles that play critical roles in neuronal polarity and migration. HSPA6 and HSPA1A also targeted nuclear speckles, rich in RNA splicing factors, and the granular component of the nucleolus that is involved in rRNA processing and ribosomal subunit assembly. HSPA6 and HSPA1A displayed similar FRAP kinetics in their interaction with nuclear speckles and the nucleolus. Subsequently, during the recovery from neuronal stress, HSPA6, but not HSPA1A, localized with the periphery of nuclear speckles (perispeckles) that have been characterized as transcription sites. The stress-induced association of HSPA6 with perispeckles displayed the greatest dynamism compared to the interaction of HSPA6 or HSPA1A with other stress-sensitive cytoplasmic and nuclear structures. This suggests involvement of HSPA6 in transcriptional recovery of human neurons from cellular stress that is not apparent for HSPA1A.

Electronic supplementary material

The online version of this article (doi:10.1007/s12192-016-0724-2) contains supplementary material, which is available to authorized users.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083669

DOI: 10.1007/s12192-016-0724-2
PubMed: 27527722
PubMed Central: 5083669

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells</title>
<author><name sortKey="Shorbagi, Sadek" sort="Shorbagi, Sadek" uniqKey="Shorbagi S" first="Sadek" last="Shorbagi">Sadek Shorbagi</name>
<affiliation><nlm:aff id="Aff1">Centre for the Neurobiology of Stress, Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4 Canada</nlm:aff>
<wicri:noCountry code="subfield">ON M1C 1A4 Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Brown, Ian R" sort="Brown, Ian R" uniqKey="Brown I" first="Ian R." last="Brown">Ian R. Brown</name>
<affiliation><nlm:aff id="Aff1">Centre for the Neurobiology of Stress, Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4 Canada</nlm:aff>
<wicri:noCountry code="subfield">ON M1C 1A4 Canada</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27527722</idno>
<idno type="pmc">5083669</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083669</idno>
<idno type="RBID">PMC:5083669</idno>
<idno type="doi">10.1007/s12192-016-0724-2</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000214</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000214</idno>
<idno type="wicri:Area/Pmc/Curation">000214</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000214</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000134</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000134</idno>
<idno type="wicri:Area/Ncbi/Merge">002057</idno>
<idno type="wicri:Area/Ncbi/Curation">002057</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002057</idno>
<idno type="wicri:doubleKey">1355-8145:2016:Shorbagi S:dynamics:of:the</idno>
<idno type="wicri:Area/Main/Merge">000377</idno>
<idno type="wicri:Area/Main/Curation">000376</idno>
<idno type="wicri:Area/Main/Exploration">000376</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells</title>
<author><name sortKey="Shorbagi, Sadek" sort="Shorbagi, Sadek" uniqKey="Shorbagi S" first="Sadek" last="Shorbagi">Sadek Shorbagi</name>
<affiliation><nlm:aff id="Aff1">Centre for the Neurobiology of Stress, Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4 Canada</nlm:aff>
<wicri:noCountry code="subfield">ON M1C 1A4 Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Brown, Ian R" sort="Brown, Ian R" uniqKey="Brown I" first="Ian R." last="Brown">Ian R. Brown</name>
<affiliation><nlm:aff id="Aff1">Centre for the Neurobiology of Stress, Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4 Canada</nlm:aff>
<wicri:noCountry code="subfield">ON M1C 1A4 Canada</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Cell Stress & Chaperones</title>
<idno type="ISSN">1355-8145</idno>
<idno type="eISSN">1466-1268</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Heat shock proteins (Hsps) are cellular repair agents that counter the effects of protein misfolding that is a characteristic feature of neurodegenerative diseases. HSPA1A (Hsp70–1) is a widely studied member of the HSPA (Hsp70) family. The little-studied HSPA6 (Hsp70B’) is present in the human genome and absent in mouse and rat; hence, it is missing in current animal models of neurodegenerative diseases. Differentiated human neuronal SH-SY5Y cells were employed to compare the dynamics of the association of YFP-tagged HSPA6 and HSPA1A with stress-sensitive cytoplasmic and nuclear structures. Following thermal stress, live-imaging confocal microscopy and <italic>F</italic>
luorescence <italic>R</italic>
ecovery <italic>A</italic>
fter <italic>P</italic>
hotobleaching (FRAP) demonstrated that HSPA6 displayed a prolonged and more dynamic association, compared to HSPA1A, with centrioles that play critical roles in neuronal polarity and migration. HSPA6 and HSPA1A also targeted nuclear speckles, rich in RNA splicing factors, and the granular component of the nucleolus that is involved in rRNA processing and ribosomal subunit assembly. HSPA6 and HSPA1A displayed similar FRAP kinetics in their interaction with nuclear speckles and the nucleolus. Subsequently, during the recovery from neuronal stress, HSPA6, but not HSPA1A, localized with the periphery of nuclear speckles (perispeckles) that have been characterized as transcription sites. The stress-induced association of HSPA6 with perispeckles displayed the greatest dynamism compared to the interaction of HSPA6 or HSPA1A with other stress-sensitive cytoplasmic and nuclear structures. This suggests involvement of HSPA6 in transcriptional recovery of human neurons from cellular stress that is not apparent for HSPA1A.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1007/s12192-016-0724-2) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Brown, Ian R" sort="Brown, Ian R" uniqKey="Brown I" first="Ian R." last="Brown">Ian R. Brown</name>
<name sortKey="Shorbagi, Sadek" sort="Shorbagi, Sadek" uniqKey="Shorbagi S" first="Sadek" last="Shorbagi">Sadek Shorbagi</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000376 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000376 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Canada
   |area=    ParkinsonCanadaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:5083669
   |texte=   Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:27527722" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a ParkinsonCanadaV1

This area was generated with Dilib version V0.6.29.
Data generation: Thu May 4 22:20:19 2017. Site generation: Fri Dec 23 23:17:26 2022

La maladie de Parkinson au Canada (serveur d'exploration)

Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells

Dynamics of the association of heat shock protein HSPA6 (Hsp70B’) and HSPA1A (Hsp70–1) with stress-sensitive cytoplasmic and nuclear structures in differentiated human neuronal cells

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	La maladie de Parkinson au Canada (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.